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  Indian J Med Microbiol
 

Figure 2: Untreated nasal S.M., D.S.M, and A.T.M. tissue samples were stained and analyzed. (a) With H and E staining, the nuclei of the cells were clearly stated in the “S. M.” samples. Cells were found eliminated after the decellularization protocol and no signs of cellular content were found in “D.S.M.” samples. Staining of the “A.T.M.” sample showed nuclei of the cells and cell clusters integrating to the scaffold. (b) The Alcian blue is envisioned for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins). “S.M.” was found highly positive for Alcian blue staining, whereas “D.S.M.” was detected fully negative. In addition, “A.T.M.” was found positive especially in specified areas. (c) The samples were stained with both Alcian blue and Alizarin red for indicating chondrogenic and osteogenic clusters. “S.M.” was found carrying positive sections for Alcian blue or Alizarin red and occasionally positive for both. On the other hand, the results were indicating insignificant staining in “D.S.M” and “A.T.M” groups. S.M.: Septal mucosa, D.S.M.: Decellularized nasal septal mucosa, A.T.M.: Artificial tympanic membrane

Figure 2: Untreated nasal S.M., D.S.M, and A.T.M. tissue samples were stained and analyzed. (a) With H and E staining, the nuclei of the cells were clearly stated in the “S. M.” samples. Cells were found eliminated after the decellularization protocol and no signs of cellular content were found in “D.S.M.” samples. Staining of the “A.T.M.” sample showed nuclei of the cells and cell clusters integrating to the scaffold. (b) The Alcian blue is envisioned for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins). “S.M.” was found highly positive for Alcian blue staining, whereas “D.S.M.” was detected fully negative. In addition, “A.T.M.” was found positive especially in specified areas. (c) The samples were stained with both Alcian blue and Alizarin red for indicating chondrogenic and osteogenic clusters. “S.M.” was found carrying positive sections for Alcian blue or Alizarin red and occasionally positive for both. On the other hand, the results were indicating insignificant staining in “D.S.M” and “A.T.M” groups. S.M.: Septal mucosa, D.S.M.: Decellularized nasal septal mucosa, A.T.M.: Artificial tympanic membrane