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  Indian J Med Microbiol
 

Figure 1: In initial attempts to isolate tragus chondrocytes, tragal cartilage was obtained and cell suspensions were generated by mincing and without using enzymatic digestion. Isolated cells were seeded on polystyrene-coated dishes. (a) Although being propagated in vitro, the number of tragus chondrocytes was found to be low before incubation with Collagenase I and Collagenase IV. (b) After incubating the tragal cartilage samples with Collagenase I and Collagenase IV for 2 h at 37°C, the chondrocytes were found regular and better proliferating

Figure 1: In initial attempts to isolate tragus chondrocytes, tragal cartilage was obtained and cell suspensions were generated by mincing and without using enzymatic digestion. Isolated cells were seeded on polystyrene-coated dishes. (a) Although being propagated <i>in vitro</i>, the number of tragus chondrocytes was found to be low before incubation with Collagenase I and Collagenase IV. (b) After incubating the tragal cartilage samples with Collagenase I and Collagenase IV for 2 h at 37°C, the chondrocytes were found regular and better proliferating