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 Table of Contents  
CASE REPORT
Year : 2012  |  Volume : 18  |  Issue : 2  |  Page : 103-105

Chronic fungal rhinosinusitis due to Aspergillus versicolor


1 Department of Microbiology, Smt Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India
2 Smt Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India

Date of Web Publication6-Sep-2012

Correspondence Address:
Rupali J Mali
Department of Microbiology, Smt Kashibai Navale Medical College and General Hospital, S. No. 49/1 and 53/2 Narhe, Off Mumbai-Pune Westerly Bypass, Pune 411 041, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0971-7749.100741

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  Abstract 

Fungal rhinosinusitis is an increasingly recognized entity in both immunocompromised as well as immunocompetent patients. Aspergillus spp. is commonest of all causative fungi. A case of chronic fungal rhinosinusitis in a 35-year-old immunocompetent female is reported. Presenting symptoms were bilateral nasal block, discharge, and change of voice. Initially, it was diagnosed as nasal polyposis. Microscopy and culture established the diagnosis of chronic fungal rhinosinusitis due to Aspergillus versicolor.

Keywords: Aspergillus versicolor, Fungal rhinosinusitis, immunocompetent host


How to cite this article:
Ghadage DP, Mali RJ, Wankhade AB, Bhore AV. Chronic fungal rhinosinusitis due to Aspergillus versicolor. Indian J Otol 2012;18:103-5

How to cite this URL:
Ghadage DP, Mali RJ, Wankhade AB, Bhore AV. Chronic fungal rhinosinusitis due to Aspergillus versicolor. Indian J Otol [serial online] 2012 [cited 2019 Jul 21];18:103-5. Available from: http://www.indianjotol.org/text.asp?2012/18/2/103/100741


  Introduction Top


Fungal rhinosinusitis refers to a spectrum of disease ranging from benign colonization of the nose and sinuses by pathogenic fungi to acute invasive and fatal inflammation extending to the orbit and brain. The disease causes high morbidity and high mortality if misdiagnosed. Most common fungi causing fungal rhinosinusitis are Aspergillus fumigatus and Aspergillus flavus. [1] A. versicolor is rarely encountered as a human pathogen. An association of nasal polyposis and Aspergillus rhinosinusitis has been noted. Here, a case of Aspergillus versicolor rhinosinusitis in an immunocompetent patient is reported.


  Case Report Top


A 35-year-old housewife presented with history of nasal block, nasal discharge, and sneezing, associated with change of voice, since 5-6 months. She had complaints of repeated episodes of nasal obstruction for the past 1 year with intermittent regression of symptoms. Onset was insidious, starting on the left side, which gradually progressed to bilateral nasal block. She complained of scanty, greenish, odorless nasal discharge. There was no history of trauma to nose, bleeding, headache, or facial pain. No history of diabetes mellitus, asthma, use of corticosteroids, and prolonged antibiotic therapy. On general examination, vital parameters were within normal range. No facial disfigurement or swelling was observed. Anterior rhinoscopy revealed bilateral sinonasal polyps. Scanty greenish discharge was seen. There was no abnormal finding on posterior rhinoscopy.

Laboratory investigations like hemoglobin and blood sugar levels were within normal range. Eosinophil count was 0.3%. The patient tested negative for HIV. CT scan of paranasal sinuses showed opacity in left and right maxillary sinus. Evidence of polypoidal sinusitis of left maxillary, ethmoid, sphenoid, and frontal sinuses was reported. The patient was posted for functional endoscopic sinus surgery (FESS). Cheesy, greenish black dirty tissue was removed and suction was done.

Tissue material from the left nostril was received for fungal culture. KOH (10%) mount showed pus cells with hyaline, septate hyphae with acute-angled branching. The specimen was inoculated on Sabouraud dextrose agar (SDA) with and without antibiotics and incubated at 37°C. On 8 th day of incubation, obverse showed small pink-to-flesh-colored velvety colonies. On successive days of incubation, colony color changed from orange-yellow to green, and reverse of equally variable color [Figure 1].
Figure 1: (a) Colony of Aspergillus versicolor on Sabouraud dextrose agar (SDA) (obverse), (b) Colony of Aspergillus versicolor on SDA (reverse)

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In lactophenol cotton blue (LPCB) tease mount of colonies, septate and hyaline hyphae with acute-angled branching were seen. Small conidial heads, consisting of an ovoid vesicle bearing metulae below the layer of phialides, were seen; reduced conidial structures resembling those of Penicillium species were present. These features were suggestive of A. versicolor [Figure 2]. Histopathological examination of tissue showed fungal hyphae with granulomatous inflammation. The patient was started with antifungal treatment.
Figure 2: Lactophenol cotton blue mount of colony on Sabouraud dextrose agar

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  Discussion Top


Fungal rhinosinusitis, once considered a rare disorder, is now being reported with increasing frequency worldwide. Now in India, this disease is not only prevalent in northern regions, but also is reported from other parts of the country. [2] In Sudan, Indian subcontinent, and other tropical areas, cases of Aspergillus invasive disease of paranasal sinuses in immunocompetent patients have frequently been reported. [3]

The patient was presented with bilateral nasal stuffiness. Alrajhi et al., in their study reported nasal obstruction as the most common presenting symptom (87%). Patients with anatomic abnormalities of the paranasal sinuses that impair drainage, like nasal polyps, are vulnerable to fungal colonization. [3] Examination showed bilateral sinonasal polyps. Telmesani reported 12.1% (11/91) incidence of allergic fungal sinusitis among patients with nasal polyps. [4]

CT scan of the patient showed multiple sinus involvement with marked opacity in left and right maxillary sinus. In study by Alrajhi et al., abnormalities of paranasal sinuses were noted on CT scan in all patients; all sinuses were involved in 61% of patients. [3]

At FESS, cheesy, greenish black dirty tissue was removed, which is a common finding in fungal rhinosinusitis cases. [5] Aspergillus spp. are commonly isolated from fungal paranasal sinusitis cases. Other causes include several phaeoid mycelial fungi, zygomycetes. [1]

Demonstration of Aspergillus spp. by both culture and microscopy provides the most firm diagnosis. [6] In the present study, findings of KOH mount, variable color changes of colony on SDA, and characteristic structure on LPCB was suggestive of Aspergillus versicolor. [7]

Although Aspergillus versicolor is not a common cause of invasive aspergillosis, there are case studies in which Aspergillus versicolor is reported as causal agent of cerebral abscess [8] and endogenous endophthalmitis in immunocompetent patient. [9]

Hedayati et al., in a study of chronic fungal rhinosinusitis demonstrated that using a standard mycology laboratory protocol, which is relatively inexpensive and readily available, fungus can be isolated from a majority of patients undergoing sinus surgery. [6]

In our case, Aspergillus versicolor was isolated, which is an infrequent human pathogen. As it is a rare cause, further study and identification at the molecular level is necessary. By reporting this case, we want to suggest that diagnosis of aspergillosis of the paranasal sinuses requires a high index of suspicion, which should be present, particularly in patients with nasal polyposis. Early diagnosis is necessary in order to avoid destructive disease and to start early treatment before irreversible condition arises.

 
  References Top

1.Chatterjee SS, Chakrabarti A. Epidemiology and medical mycology of fungal rhinosinusitis. Otorhinolaryngol Clin: An Int J 2009;1:1-13.  Back to cited text no. 1
    
2.Chakrabarti A, Das A, Panda NK. Overview of fungal rhinosinusitis. Indian J Otolaryngol Head Neck Surg 2004;56:251-8.  Back to cited text no. 2
    
3.Alrajhi AA, Enani M, Mahasin Z, Al-Omran K. Chronic invasive aspergillosis of the paranasal sinuses in immunocompetent hosts from Saudi Arabia. Am J Trop Med Hyg 2001;65:83-6.  Back to cited text no. 3
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4.Telmesani LM. Prevalence of allergic fungal sinusitis among patients with nasal polyps. Ann Saudi Med 2009;29:212-4.   Back to cited text no. 4
[PUBMED]  Medknow Journal  
5.Agarwal S, Kanga A, Sharma V, Sharma DR, Sharma ML. Invasive aspergillosis involving multiple paranasal sinuses - A case report. Indian J Med Microbiol 2005;23:195-7.  Back to cited text no. 5
[PUBMED]  Medknow Journal  
6.Hedayati MT, Bahoosh M, Kasiri A, Ghasemi M, Motahhari SJ. Prevalence of fungal rhinosinusitis among patients with chronic rhinosinusitis from Iran. J Med Mycol 2010;20:298-303.  Back to cited text no. 6
    
7.Colin K, Campbell, Elizabeth M Johnson. Superficial and ocular fungal infections Part III: Dermatomycotic molds. In: Merz WG, Hay RJ, editors. Topley and Wilson's Microbiology and Microbial Infections 10 th ed. USA: ASM press; 2005. p. 249-50.  Back to cited text no. 7
    
8.Venugopal PV, Taralaxmi V, Thiruneeklakantan K, Subramanian S, Shetty BMV. Cerebral aspergillosis: Report of two cases. Med Mycol 1977;15:225-30.  Back to cited text no. 8
    
9.Perri P, Campa C, Incorvaia C, Parmeggiani F, Lamberti G, Costagliola C. Endogenous Aspergillus versicolor endophthalmitis in an immuno-competent HIV-positive patient. Mycopathologia 2005;160:259-61.  Back to cited text no. 9
    


    Figures

  [Figure 1], [Figure 2]



 

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